Any non-specific bands from the original image should be included in the figure and explained in the text or figure legend. Figure panels should include some background area above and below bands.
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Please follow these instructions when preparing and submitting blot/gel data files:
Whilst it is not necessary to provide original images at time of initial submission, we will require these files during the peer review process or before a manuscript can be accepted. If you have questions about these requirements please email us at Original images for blots and gelsĪuthors must provide the original, uncropped and minimally adjusted images supporting all blot and gel results reported in an article’s figures and supporting information files. The figure preparation guidelines that follow clarify PLOS ONE standards and requirements, and aim to ensure the integrity and scientific validity of blot/gel data reporting.
The original images also provide additional information for readers about background within the experiment and the specificity of reagents used. The underlying data requirement is in place to ensure that the results are reported in a fully transparent manner, and that readers can verify results by reviewing the primary data in its original form. The following requirements apply to any figures and supporting Information files that report blot or gel data.